Handling Normalization Errors and Warnings
WARNING – AF for led X out of bounds!
Observe each Attenuation Factor (AF) warning. Review each AF warning against the table below and press ENTER to continue past each warning.
The reader should be replaced if any AF value for and LED exceeds the Upper Maximum for that LED in the table below.
Table: Optical Channel AF Limit
| Optic Channel | LED Number | Upper AF Limit |
|---|---|---|
| ROX | 0 and 6 | 2.00 |
| Cy5 | 2 and 8 | 2.00 |
| FAM | 3 and 9 | 1.50 |
| CY5.5 | 4 and 10 | 2.00 |
| VIC | 5 and 11 | 1.50 |
WARNING – N for led X out of bounds!
Look at each normalizer value (NV) warning. If the normalizer value has fallen below the lower Limit or higher limit, accept and proceed with the normalization (The normalization will correct the values to the normal). pay extra attention to the subsequent qualification runs. Then, observe the qualification runs for EVALs due to a color channel being too noisy.
Linearity Warning
Linearity warning can either be caused by loose hardware, bad control board, bad home sensor, or dirty normalizer card/tray. If Linearity Warning occurs, clean the tray and normalizer card, properly secure all cable connections, and then try renormalizing again. If the same warning appears again, then replace the reader. If the error does not return, then the reader can be left as is and proceed with qualification.